The history of both theater and knowledge are intertwined, and anatomy is part of the general history of the stage. These two assertions are linked by one of anatomy’s most relevant features, namely the anatomical practice in Early Modern Europe which illustrated the way in which performance shaped modes of knowledge. It is indeed in the anatomy of Early Modern Europe that a knowledge based- project on performativity was implemented. Through the public dissection of a dead body, a theoretical discourse on the epistemological role of the senses, the invention of specific spaces designed for public shows, anatomical theatres, and a series of preset actions, we realize that the staging of all these events are closely associated.